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KMID : 0545120220320010081
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Journal of Microbiology and Biotechnology
2022 Volume.32 No. 1 p.81 ~ p.90
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Effects of 3′-isovaleryl-4′-senecioylkhellactone from Peucedanum japonicum Thunberg on PMA-Stimulated Inflammatory Response in A549 Human Lung Epithelial Cells
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Hwang Da-Seul
Ryu Hyung-Won
Park Ji-Won
Kim Jung-Hee
Kim Doo-Young
Oh Jae-Hoon
Kwon Ok-Kyoung
Han Sang-Bae
Ahn Kyung-Seop
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Abstract
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Peucedanum japonicum Thunberg (PJT) has been used in traditional medicine to treat colds, coughs, fevers, and other inflammatory diseases. The goal of this study was to investigate whether 3′-isovaleryl-4′-senecioylkhellactone (IVSK) from PJT has anti-inflammatory effects on lung epithelial cells. The anti-inflammatory effects of IVSK were evaluated using phorbol 12-myristate 13-acetate (PMA)-stimulated A549 cells and regular human lung epithelial cells as a reference. IVSK reduced the secretion of the inflammatory mediators interleukin (IL)-8 and monocyte chemoattractant protein-1 (MCP-1), and the mRNA expression of IL-6, IL-8, MCP-1, and IL-1β. Additionally, it inhibited the phosphorylation of IκB kinase (IKK), p65, Iκ-Bα, and mitogen-activated protein kinases (MAPKs) p38, JNK, and ERK in A549 cells stimulated with PMA. Moreover, the binding affinity of activator protein-1 (AP-1) and nuclear factor-κB (NF-κB) was significantly reduced in the luciferase assay, while nuclear translocation was markedly inhibited by IVSK in the immunocytochemistry. These findings indicate that IVSK can protect against inflammation through the AP-1 and NF-κB pathway and could possibly be used as a lead compound for the treatment of inflammatory lung diseases.
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KEYWORD
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3′-isovaleryl-4′-senecioylkhellactone, Peucedanum japonicum Thunberg, inflammation, NF-κB, AP-1
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